American Dad! (2005)

S17E18 The Old Country

Steve is curious about the Smith family lineage, but Stan dismisses his inquiries, fixated on his pride in being American. Insistent on uncovering the truth, Steve secretly collects a DNA sample from Stan to conduct a home test. The results reveal that they are actually Canadian. While Steve celebrates his newfound heritage, Stan is crushed by the revelation, feeling his identity has been undermined.